Correlation analysis between effectiveness of element in rhizonsphere soil and quality of Danshen (Salvia miltiorrhiza) / 中国中药杂志

In order to investigate the content and distribution of available element in the rhizonsphere soil of the growing areas of Salvia miltiorrhiza Bunge, the contents of available element (N,P,K,B,Cu,Zn,Fe,Mn) in 26 soil samples were tested and evaluated. The results showed that the contents of available P and Fe were very plentiful, available K, Cu and Zn were rich, available N and Mn were deficient, available B was extremely deficient in all growing areas of S. miltiorrhiza of eight provinces in China. The correlation analysis showed that the contents of eight kinds of available elements were varying degree correlation. The stepwise regression analysis between the contents of available elements of rhizonsphere soil and ten kinds of active ingredients of Danshen (Salviae Miltiorrhizae Radix et Rhizoma) were researched. The results showed that the rates of contribution of available N,B,Mn and Fe to quality of Danshen were relatively large and they were the significant factors, and the other factors did not show statistical significance. The recommended fertilizing strategies is that the usage of N,B and Mn fertilizers should be controlled according to different stages of growth of S. miltiorrhiza, and P fertilizer should be reduced in all growing areas of S. miltiorrhiza.

Preparation and characterization of ginsenoside-Rh₂ lipid nanoparticles and synergistic effect with borneol in resisting tumor activity / 中国中药杂志

To prepare ginsenoside-Rh₂ lipid nanoparticles, and investigate the synergistic effect with borneol in resisting tumor activity in vitro. Ginsenoside-Rh₂ lipid nanoparticles were prepared by ultrasonic-assisted solvent evaporation method, and orthogonal design was adopted to optimize formulation process. Its encapsulation efficiency, drug loading ratio, particle size distribution, Zeta potential, morphology and in vitro drug release behavior were characterized, and synergistic effect with borneol in resisting tumor activity were preliminarily studied by MTT. These nanoemulsion particles prepared by the optimized process method were rounding and even in a good shape. Encapsulation efficiency and drug loading ratio of three batches of nanoemulsion particles were (77.3±2.5)% and (7.2±0.2)%, respectively. Nanoemulsion particles showed an obvious sustained release characteristics, with 52.42% cumulative release within 96 h. The killing effect of nanoemulsion particles on glioma cells was dose-dependent, with IC₅₀ of 22.33 μmol•L⁻¹ and 11.46 μmol•L⁻¹ after 24 h and 48 h, respectively. After the combination with borneol, the killing effect of nanoemulsion particles on glioma cells was dose-dependent, with IC₅₀ of 16.36 μmol•L⁻¹ and 8.04 μmol•L⁻¹ after 24 h and 48 h, respectively.

Isolation and identification of endophytic fungi from Ligusticum / 中国中药杂志

<p><b>OBJECTIVE</b>To investigate the correlation between entophytic fungal community, habitations and varieties of Ligusticum.</p><p><b>METHOD</b>The solidified potato dextrose agar (PDA) of plates was applied for the isolation of the endophytic fungi, and the identification was completed by spot-planting method.</p><p><b>RESULT</b>Fifty strains of the entophytic fungi were isolated from the rhizome of L. chuanxiong collected from 6 habitations. They were morphologically identified as belonging to 13 genera, 4 families, 3 orders and 1 class.</p><p><b>CONCLUSION</b>There were some differences at quantity, species and distributing of the entophytic fungi in different habitats and varieties of Ligusticum, which was suggested that entophytic fungal community is related with specific habitat.</p>